O-Level Biology Food Tests & Reagents Playbook (Paper 3)
02 Nov 2025, 00:00 Z
Reviewed by
Ezekiel Tan·Academic Advisor (Biology)
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TL;DR
Paper 3 often revisits food-test and reagent-led investigations, and the 2026 syllabus provides a guidance list of typical apparatus and materials (the exact exam requirements vary year-on-year and centres are notified in advance).
Drill reagent prep, observation tables, and risk controls now so MMO, PDO, and ACE strands stay intact even when the task throws a twist (e.g. modifications or follow-up hypotheses).
1 | Reagents SEAB expects every lab to stock
- The 2026 O-Level Biology syllabus (6093) includes a guidance list of common reagents and materials (e.g., iodine, Benedict's, ethanol, biuret reagent, hydrogencarbonate indicator, methylene blue, petroleum jelly) that centres are generally expected to have available (SEAB).
- Assume each bottle arrives with a dropper pipette unless told otherwise. Your plan should state if a clean dropper is needed to avoid cross-contamination.
- Label every reagent in your table with concentration or state (e.g. Benedict's solution, biuret reagent, hydrogencarbonate indicator). Leaving this blank invites PDO penalties for vague observations.
2 | Food-test sequence without lost time
| Target | Reagent sequence | Observation cues | Skill checkpoints (P/MMO/PDO/ACE) |
| Reducing sugars | Use a clean test tube, add |
