H2 Biology Practical: Ecology and Fieldwork Sampling Guide
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Q: What does H2 Biology Practical: Ecology and Fieldwork Sampling Guide cover?
A: Build Paper 4-ready ecology and fieldwork practicals with sampling plans, chi-squared-ready data tables, and clear MMO/PDO/ACE write-ups aligned with the SEAB syllabus.
TL;DR
Treat ecology practicals as data-quality exercises: define your sampling strategy, record raw counts clearly, and structure tables so a chi-squared test can be applied quickly. Use consistent sampling intervals, document any sources of bias, and show how your data supports the conclusion.
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Status: SEAB H2 Biology (9477, first exam 2026) syllabus last checked 2025-12-24. The mathematical requirements state that candidates may need to present data graphically, calculate rates, and apply chi-squared tests to results from genetics or ecological sampling. [1]
1 Why ecological sampling matters in Paper 4
- The syllabus highlights ecological sampling in the context of chi-squared testing, so fieldwork data handling remains a valid practical context. [1]
- Exam scripts can include sampling data sets even when apparatus is limited, so your PDO and ACE structures still need to be strong.
- Sampling investigations test the full Paper 4 skill set: planning the sampling method, collecting repeatable observations, and evaluating limitations.
2 Sampling plan: build your data before you step outside
- Define the sampling question. Example: compare species frequency across two habitats or treatment zones.
- Choose a sampling frame. Use a consistent area or interval so that comparisons are valid.
- Set the sample size. Decide on the number of sampling points and document why this reduces bias.
- List control variables. Keep the time of sampling, observation duration, and equipment constant.
If your school uses quadrats or transects for sampling, ensure the interval spacing and area are recorded clearly so your analysis is transparent.
3 Data tables that prepare for chi-squared analysis (PDO)
- Raw count table: list each sampling point and the raw counts by species or category.
- Totals table: sum counts across categories and calculate expected values if needed.
- Graphical check: use a bar chart or stacked plot to visualise patterns before running the chi-squared test.
The syllabus notes that chi-squared tests are used to evaluate ecological sampling results. [1]




