H2 Biology Catalase Practical: Enzyme Kinetics Gas-Syringe Guide
12 Oct 2025, 00:00 Z
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Practical course certificate note
For practical, lab, and experiment courses, Eclat Institute may issue an internal Certificate of Completion/Attendance based on participation and internal assessment.
- This is an internal centre-issued certificate, not an MOE/SEAB qualification or accreditation.
- Recognition (if any) is determined by the receiving school, institution, or employer.
- For SEAB private candidates taking science practical papers, SEAB states you should either have taken the subject before or complete a practical course before the practical exam date.
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Q: What does H2 Biology Practical: Enzyme Kinetics (Catalase) Guide cover?
A: Run a syllabus-aligned catalase assay with serial dilutions, temperature control, initial rate estimation, and spreadsheet-backed graphs so MMO/PDO/ACE marks are predictable.
TL;DR
Treat enzyme practicals as controlled investigations: set a single independent variable, keep conditions constant, record volumes/times consistently, and calculate initial rate from the linear region. Use a clean serial dilution, control temperature, and graph rate vs substrate concentration with error bars for ACE credit.
Status: SEAB H2 Biology (9477, first exam 2026) syllabus and Scheme of Assessment last checked 2025-11-29 (PDF last modified 2025-11-28). Paper 4 remains 2 h 30 min, 50 marks, with Planning at 4 % and MMO/PDO/ACE at 16 % combined; enzyme rate investigations stay within Core Idea 3’s practical expectations.
1 Aim & overview (catalase + hydrogen peroxide)
- Investigate how substrate concentration affects catalase activity using the oxygen evolved.
- Keep pH and temperature constant; measure initial rate via gas volume/time or pressure change.
- Recommended timing: 60 minutes end-to-end with one variable across 5-6 levels.
- Syllabus alignment: SEAB H2 Biology (9477, first exam 2026) expects investigation of enzyme-catalysed rates via gas output from catalase across substrate concentrations; Paper 4 carries 20% of H2 with Planning at 4% and MMO/PDO/ACE at 16% combined.
2 Apparatus & reagents
- Catalase source: yeast suspension or blended potato (filtered).
- Substrate: hydrogen peroxide standards (e.g., , ,
